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Absorbance Measurement
From UV to NIR and longer wavelengths, absorbance measurements provide valuable information about the chemical composition of materials in all states of matter.
入射在样品上的光可以传输，吸收或分散。传输是通过样品的光线;遇到分子的光可以被吸收或散射。吸光度可用作定性工具来鉴定物质，或作为测量溶液中分子浓度的定量工具。
Absorbanc的优点e
- 非破坏性测量：Unless your sample is photosensitive, the measurement can be repeated without altering the sample.
- Quantitative results:Use absorbance to determine the concentration of solutions.
- Accuracy and repeatability:With proper tools and methods, absorbance can be quantified with great measurement-to-measurement precision.
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Absorbance Video Tutorial
In this video, we demonstrate how to perform a basic absorbance measurement using Ocean Insight products. We also explain OceanView software procedures and share tips to ensure the best results.
UV Absorbance for Continuous, Online Monitoring of Proteins
Learn more about continuous, online monitoring of proteins using UV absorbance. See how we use an Ocean HDX high-sensitivity spectrometer to capture real-time protein concentration values, and then convert the data into answers that are meaningful to the user.
Absorbance of Solutions
When configuring a setup for absorbance measurements, your first question should be what you hope to accomplish. Is this a system for general lab use? For teaching lab purposes? For monitoring a manufacturing or testing process?
With your outcome in mind, here’s some good news: Ocean Insight has the tools and expertise to help you configure spectral systems for hundreds of different absorbance applications.
Consider the setup in the illustration. Configuring a system for general-purpose absorbance begins with a spectrometer like theOcean HDXor theFlame，两者都覆盖了UV-VIS范围，或NIRQuest+for the NIR. Add a light source – we’re showing adeuterium-halogen, which provides output across the UV, Vis and NIR – and your halfway there.
To complete the system, you’ll need a sampling setup. Acuvette holderfor 1 cm pathlength cuvettes is a great start, although for in situ measurements you’ll want atransmission dip probe. With a cuvette holder, you’ll also need an optical fiber to illuminate the sample cell and another fiber to return the signal to the spectrometer.
You may also consider adding anabsorbance standardto verify the photometric accuracy of your measurements.
The finishing touch? Set up your experiment inOceanView operating softwareusing our simple, step-by-step Absorbance Wizard.
Case Study
App Notes
Product Sheet
This 1 cm pathlength cuvette holder is ideal for accurate, repeatable absorbance and fluorescence measurements. Square One provides a snug, reliable fit for cuvettes and filters. Download Product Sheet to learn more!
